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Isolating DNA and Transformation with Plasmids Pre-Lab Worksheet

Week 6 Isolating DNA and Transformation with Plasmids Pre-Lab Worksheet (5pts) Name: Emily Aderman Section # 6 Read the lab manual information for Week 6 and the appropriate Freeman textbook sections for this week. Based on you reading, answer the following questions. 1. What is the difference between genomic DNA and plasmid DNA in bacteria? In the DNA transformation experiment that you will perform in lab during Week 6, what is the purpose of using plasmid DNA? (2 pts) Plasmid DNA is smaller and rounded, it also belongs to plasmids while genomic. DNA belongs to organism. The plasmid DNA is used because it is using them to transfer genes. 2. Which sample(s) will serve as a control for the experiment? Explain your reasoning. (1pt) The ones with no plasma will be the control. This experiment will show how the Arabinose, Ampicillin and how they act alone without the plasmid.

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